Please help! Converting ng/ml to nM

Hi guys, Im really struggling with this simple conversion. I need to convert insulin ng/ml to nanomoles. Can anyone offer any help.

my starting concentration of insulin is 3.5ng/ml which I need to convert into nM.

the mW of insulin is 5808kD

Thanks all

x

[JohnS]

Originally Posted by Unregistered

Hi guys, Im really struggling with this simple conversion. I need to convert insulin ng/ml to nanomoles. Can anyone offer any help.

my starting concentration of insulin is 3.5ng/ml which I need to convert into nM.

the mW of insulin is 5808kD

Thanks all

x

"M" as in 1 M solution is used to represent a concentration of 1 mol/L.

Also, per Wikipedia, the molecular weight is 1/1000 of the value you gave, 5808 Da or 5.808 kDa, and the molar mass is 5808 g/mol.
http://en.wikipedia.org/wiki/Insulin

3.5 ng/mL = 3.5 µg/L.
If we divide by 5808 g/mol, we get 0.603 nmol/L = 0.603 nM.

Dont have the answer, but a similar question:
How do I convert insulin 119 pmol/L to microgram/L? Anyone? =D

[JohnS]

Originally Posted by Unregistered

Dont have the answer, but a similar question:
How do I convert insulin 119 pmol/L to microgram/L? Anyone? =D

119 pmol/L x 5808 g/mol = 0.691 µg/L

Hi,

I'm having a similar problem. I nanodropped my protein (80KDa) and found it was 0.19mg/ml. I used 12ul of this protein in an experiment and need to find out the concentration I used in terms of nM?

Always remember 1Da = 1 g/mol

Everything else is easy if you keep this on mind.

covert 461 pm ol/l to ng/ml

[JohnS]

Originally Posted by Unregistered

covert 461 pm ol/l to ng/ml

The conversion is substance-specific. It depends on the molecular weight of the substance, which is numerically equal to the molar mass in grams per mole. Without it, there is no way to proceed.

I am trying to convert 5 ug/L to nM. The molecular weight of the substance is 897.1

[JohnS]

Originally Posted by Unregistered

I am trying to convert 5 ug/L to nM. The molecular weight of the substance is 897.1

5 µg/L x 1 mol/897.1 g =5.57 nmol/L = 5.57 nM