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#1
09-12-2008, 02:42 AM
 Unregistered Guest Posts: n/a
Please help! Converting ng/ml to nM

Hi guys, Im really struggling with this simple conversion. I need to convert insulin ng/ml to nanomoles. Can anyone offer any help.

my starting concentration of insulin is 3.5ng/ml which I need to convert into nM.

the mW of insulin is 5808kD

Thanks all

x
#2
09-12-2008, 03:28 PM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,441 Rep Power: 19
Re: Please help! Converting ng/ml to nM

Quote:
 Originally Posted by Unregistered Hi guys, Im really struggling with this simple conversion. I need to convert insulin ng/ml to nanomoles. Can anyone offer any help. my starting concentration of insulin is 3.5ng/ml which I need to convert into nM. the mW of insulin is 5808kD Thanks all x
"M" as in 1 M solution is used to represent a concentration of 1 mol/L.

Also, per Wikipedia, the molecular weight is 1/1000 of the value you gave, 5808 Da or 5.808 kDa, and the molar mass is 5808 g/mol.
http://en.wikipedia.org/wiki/Insulin

3.5 ng/mL = 3.5 µg/L.
If we divide by 5808 g/mol, we get 0.603 nmol/L = 0.603 nM.
#3
11-18-2008, 09:49 AM
 Unregistered Guest Posts: n/a
Re: Please help! Converting ng/ml to nM

Dont have the answer, but a similar question:
How do I convert insulin 119 pmol/L to microgram/L? Anyone? =D
#4
11-18-2008, 12:43 PM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,441 Rep Power: 19
Re: Please help! Converting ng/ml to nM

Quote:
 Originally Posted by Unregistered Dont have the answer, but a similar question: How do I convert insulin 119 pmol/L to microgram/L? Anyone? =D
119 pmol/L x 5808 g/mol = 0.691 µg/L
#5
01-06-2009, 11:00 AM
 Unregistered101 Guest Posts: n/a
Re: Please help! Converting ng to nM

Hi,

I'm having a similar problem. I nanodropped my protein (80KDa) and found it was 0.19mg/ml. I used 12ul of this protein in an experiment and need to find out the concentration I used in terms of nM?
#6
05-07-2010, 10:34 AM
 Unregistered Guest Posts: n/a
Re: Please help! Converting ng/ml to nM

Always remember 1Da = 1 g/mol

Everything else is easy if you keep this on mind.
#7
08-26-2010, 02:33 AM
 Unregistered Guest Posts: n/a
Re: Please help! Converting pmol/l to ng/ml

covert 461 pm ol/l to ng/ml
#8
08-26-2010, 03:32 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,441 Rep Power: 19
Re: Please help! Converting pmol/l to ng/ml

Quote:
 Originally Posted by Unregistered covert 461 pm ol/l to ng/ml
The conversion is substance-specific. It depends on the molecular weight of the substance, which is numerically equal to the molar mass in grams per mole. Without it, there is no way to proceed.
#9
12-08-2010, 12:45 PM
 Unregistered Guest Posts: n/a
Re: Please help! Converting ug/L to nM

I am trying to convert 5 ug/L to nM. The molecular weight of the substance is 897.1
#10
12-08-2010, 02:43 PM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,441 Rep Power: 19
Re: Please help! Converting ug/L to nM

Quote:
 Originally Posted by Unregistered I am trying to convert 5 ug/L to nM. The molecular weight of the substance is 897.1
5 µg/L x 1 mol/897.1 g =5.57 nmol/L = 5.57 nM

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