Morpholino concentrations

Hi i really need some help i'm totally confused.

Im using morpholinos i get a vial that says 300nmol i add 300ul im assuming that results in 1mM solution correct me if im wrong. if i use 1nl waht would the concentraion be in ng/ug/ul

Please help

[JohnS]

Originally Posted by Unregistered

Hi i really need some help i'm totally confused.

Im using morpholinos i get a vial that says 300nmol i add 300ul im assuming that results in 1mM solution correct me if im wrong. if i use 1nl waht would the concentraion be in ng/ug/ul

Please help

You need to know the concentration. The 300 nmol must be that amount contained in some volume, and you need to determine that volume to know the concentration. Then multiply that concentration times the volume taken to get total amount of substance.

To convert amount of substance (moles) to grams you have to know the molecular weight of the substance which is numerically equal to the molar mass in grams per moles.

The 300 nmol on the vial refers to 300 nanomoles. There is no volume associated with that measurement. You will add water to reach a certain volume, at which time you will have a solution with a particular molarity.

If you have 300 nanomoles dissolved in 300 microliters, that is a 1 milliMolar solution.

To determine what the concentration of the solution is in ng/ul, you will need the molecular mass of the Morpholino oligo (usually about 8200 Da for a 25-base Morpholino).

Some Morpholino sequences can be difficult to dissolve completely, so be sure you have the oligo completely in solution. Heating and vortexing can help if an oligo is slow to dissolve. You can find some advice on dissolution and storage here on the Gene Tools website, in the Customer Support section, on the Downloads page. Look for "Notes on storage and lyophilization of Morpholinos" in section A.

- Jon Moulton
Gene Tools, LLC