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#1
08-04-2011, 05:08 AM
 Unregistered Guest Posts: n/a
mg/ml to ug/ml dilution

I have a stock solution of 1mg/mL that i want to dilute to 2ug/ml in 10mL solvent
How do i do this?
#2
08-04-2011, 05:47 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,536 Rep Power: 19
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered I have a stock solution of 1mg/mL that i want to dilute to 2ug/ml in 10mL solvent How do i do this?
The math is easier if you use a tad less solvent and make up 10 mL total
1000 µg/mL * x mL = 2 µg/mL * 10 mL
x = 0.02 mL.
Dilute to 10 mL in volumetric flask

If you are determined to add it to a pre-measured 10 mL, assuming volumes are additive
1000 µg/mL * x mL = 2 µg/mL * (10 mL + x mL)
x = 20/998 mL = 0.02004 mL

The distinction is more important when the dilution ratios are smaller. It is largely a pedantic difference in this case.

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