Welcome to OnlineConversion.com Forums

 OnlineConversion Forums Pancreatin enzyme!! Calculation
 [ Home ] [ Forum Home ] Register FAQ Calendar Search Today's Posts Mark Forums Read

 Convert and Calculate Post any conversion related questions and discussions here. If you're having trouble converting something, this is where you should post.* Guest Posting is allowed.

#1
09-06-2010, 06:31 PM
 abzal Junior Member Join Date: Sep 2010 Posts: 2 Rep Power: 0
Pancreatin enzyme!! Calculation

Do any one can help me, how to calculate amylase in USP unit? In USP method there is a formula as following:

Amylase activity (USP unit/mg)= [(Cs/Wu)*(Vbu-Vu)/(Vbs-Vs)*100]
where
Cs: Amylase activity of standard solution (USP units/ml)
Wu = Amount of sample
Vbu= Volume of 0.1N sodium thiosulfate for blank in Test enzyme
Vu= Volume of 0.1N sodium thiosulfate for Test enzyme
Vbs= Volume of 0.1N sodium thiosulfate for blank in standard enzyme
Vs= Volume of 0.1N sodium thiosulfate for standard enzyme

However I do not know what is the factor "100" plus if there is any dilution during assay preparation then, how dilution will be accomodate?

I will appreciate very much if any one can help me,

Thanks

Abzal
#2
09-06-2010, 09:56 PM
 Mrs X can't count, can't spell! Join Date: Feb 2006 Location: New Zealand Posts: 2,296 Rep Power: 11
Re: Pancreatin enzyme!! Calculation

Quote:
 Originally Posted by abzal Do any one can help me, how to calculate amylase in USP unit? In USP method there is a formula as following: Amylase activity (USP unit/mg)= [(Cs/Wu)*(Vbu-Vu)/(Vbs-Vs)*100] where Cs: Amylase activity of standard solution (USP units/ml) Wu = Amount of sample Vbu= Volume of 0.1N sodium thiosulfate for blank in Test enzyme Vu= Volume of 0.1N sodium thiosulfate for Test enzyme Vbs= Volume of 0.1N sodium thiosulfate for blank in standard enzyme Vs= Volume of 0.1N sodium thiosulfate for standard enzyme However I do not know what is the factor "100" plus if there is any dilution during assay preparation then, how dilution will be accomodate? I will appreciate very much if any one can help me, Thanks Abzal
Hi, your volumes are in mL, and your weights are in mg. The *100 is to make it a percent, - is that what the "P" stands for in "USP"?

As for dilution, IF your diluent doesn't take part in the reaction, AND is in the reaction vessel, then it is not changing the amount of material of interest that is present.

#3
09-07-2010, 03:58 PM
 abzal Junior Member Join Date: Sep 2010 Posts: 2 Rep Power: 0
Re: Pancreatin enzyme!! Calculation

Quote:
 Originally Posted by Mrs X Hi, your volumes are in mL, and your weights are in mg. The *100 is to make it a percent, - is that what the "P" stands for in "USP"? As for dilution, IF your diluent doesn't take part in the reaction, AND is in the reaction vessel, then it is not changing the amount of material of interest that is present. Please ask again if this is not clear.

Thanks!
Are you sure 100 is the percent? P in USP is pharmacopeia and USP = United State Pharmacopeia
#4
09-08-2010, 12:02 AM
 Mrs X can't count, can't spell! Join Date: Feb 2006 Location: New Zealand Posts: 2,296 Rep Power: 11
Re: Pancreatin enzyme!! Calculation

Quote:
 Originally Posted by abzal Thanks! Are you sure 100 is the percent? P in USP is pharmacopeia and USP = United State Pharmacopeia
Yes, definitely %. You are looking at how good or effective your test enzyme is compared to your standard enzyme. The "x100" gives this ratio in percent.

Are you a student? - Hope you are enjoying your course. (I don't know anything about United State Pharmacopeia, sorry.)

 Thread Tools Display Modes Linear Mode

 Posting Rules You may post new threads You may post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Forum Rules
 Forum Jump User Control Panel Private Messages Subscriptions Who's Online Search Forums Forums Home Main Forums     Convert and Calculate     Resources     General Chat

All times are GMT -8. The time now is 09:54 AM.