Pancreatin enzyme!! Calculation

[abzal]

Do any one can help me, how to calculate amylase in USP unit? In USP method there is a formula as following:

Amylase activity (USP unit/mg)= [(Cs/Wu)*(Vbu-Vu)/(Vbs-Vs)*100]
where
Cs: Amylase activity of standard solution (USP units/ml)
Wu = Amount of sample
Vbu= Volume of 0.1N sodium thiosulfate for blank in Test enzyme
Vu= Volume of 0.1N sodium thiosulfate for Test enzyme
Vbs= Volume of 0.1N sodium thiosulfate for blank in standard enzyme
Vs= Volume of 0.1N sodium thiosulfate for standard enzyme

However I do not know what is the factor "100" plus if there is any dilution during assay preparation then, how dilution will be accomodate?

I will appreciate very much if any one can help me,

Thanks

Abzal

[Mrs X]

Originally Posted by abzal

Do any one can help me, how to calculate amylase in USP unit? In USP method there is a formula as following:

Amylase activity (USP unit/mg)= [(Cs/Wu)*(Vbu-Vu)/(Vbs-Vs)*100]
where
Cs: Amylase activity of standard solution (USP units/ml)
Wu = Amount of sample
Vbu= Volume of 0.1N sodium thiosulfate for blank in Test enzyme
Vu= Volume of 0.1N sodium thiosulfate for Test enzyme
Vbs= Volume of 0.1N sodium thiosulfate for blank in standard enzyme
Vs= Volume of 0.1N sodium thiosulfate for standard enzyme

However I do not know what is the factor "100" plus if there is any dilution during assay preparation then, how dilution will be accomodate?

I will appreciate very much if any one can help me,

Thanks

Abzal

Hi, your volumes are in mL, and your weights are in mg. The *100 is to make it a percent, - is that what the "P" stands for in "USP"?

As for dilution, IF your diluent doesn't take part in the reaction, AND is in the reaction vessel, then it is not changing the amount of material of interest that is present.

Please ask again if this is not clear.

[abzal]

Originally Posted by Mrs X

Hi, your volumes are in mL, and your weights are in mg. The *100 is to make it a percent, - is that what the "P" stands for in "USP"?

As for dilution, IF your diluent doesn't take part in the reaction, AND is in the reaction vessel, then it is not changing the amount of material of interest that is present.

Please ask again if this is not clear.

Thanks!
Are you sure 100 is the percent? P in USP is pharmacopeia and USP = United State Pharmacopeia

[Mrs X]

Originally Posted by abzal

Thanks!
Are you sure 100 is the percent? P in USP is pharmacopeia and USP = United State Pharmacopeia

Yes, definitely %. You are looking at how good or effective your test enzyme is compared to your standard enzyme. The "x100" gives this ratio in percent.

Are you a student? - Hope you are enjoying your course. (I don't know anything about United State Pharmacopeia, sorry.)