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#1
07-05-2010, 08:34 AM
 Unregistered Guest Posts: n/a
Convert microg/ml to molar concentration

Hi,

I have a protein soln at a concentration of 59 microg/ml. The MW of the protein is 59kDa, how do I calculate the molar concentration??

Thanks
#2
07-05-2010, 09:26 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,439 Rep Power: 19
Re: Convert microg/ml to molar concentration

Quote:
 Originally Posted by Unregistered Hi, I have a protein soln at a concentration of 59 microg/ml. The MW of the protein is 59kDa, how do I calculate the molar concentration?? Thanks
Since molar concentrations are expressed per liter, it is easier to consider 59 µg/mL as 59 mg/L.

The molecular weight of 59 kDa means the molar mass is 59 kg/mol

59 mg/L x 1 mol/59 kg = 1 µmol/L (micromole per liter)
#3
07-06-2010, 12:35 AM
 Unregistered Guest Posts: n/a
Re: Convert microg/ml to molar concentration

Thank You!!!!!
#4
10-16-2010, 07:00 AM
 Unregistered Guest Posts: n/a
Re: Convert microg/ml to molar concentration

Quote:
 Originally Posted by Unregistered Hi, I have a protein soln at a concentration of 59 microg/ml. The MW of the protein is 59kDa, how do I calculate the molar concentration?? Thanks
I have a protein soln at a concentration of 899.85 microg/ml. The MW of the protein is 3362649.6 kDa, how do I calculate the molar concentration??
#5
10-16-2010, 03:52 PM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,439 Rep Power: 19
Re: Convert microg/ml to molar concentration

Quote:
 Originally Posted by Unregistered I have a protein soln at a concentration of 899.85 microg/ml. The MW of the protein is 3362649.6 kDa, how do I calculate the molar concentration??
Molar concentration is moles per liter
899.85 µg/mL is also 899.95 mg/L

The molar mass is numerically equal to the molecular weight, which seems unusually high. You may wish to double check the number and units. Assum ing it is correct, the molar mass is 3362649.6 kg/mol

899.5 mg/L x 1 mol/3362649.6 kg = 267.5 pmol/L
#6
08-08-2011, 10:11 PM
 from asia Guest Posts: n/a
Re: Convert microg/ml to molar concentration

hello there

hope to help me with this one

i have 3.22 microgram/ml of drug (380 MW (a.m.u)) , how am i going to convert to microgram/M

hope to hear from u soon

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