increase dna concentration and convert from ng/uL to ug/uL

[kellekat]

Hello,
I have a DNA sample that is 660.92 ng/uL and I need the total amount of DNA per reaction to be 5 ug. The total volume of the reaction is 25uL. I believe I can just increase the amount of DNA that I use but don't know the right calculation. Would appreciate any help.
Kelly

[JohnS]

Originally Posted by kellekat

Hello,
I have a DNA sample that is 660.92 ng/uL and I need the total amount of DNA per reaction to be 5 ug. The total volume of the reaction is 25uL. I believe I can just increase the amount of DNA that I use but don't know the right calculation. Would appreciate any help.
Kelly

Well 5 µg = 5000 ng, so
5000 ng x 1 µL/660.92 ng = 7.565 µL.