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  #1  
Old 05-26-2009, 10:14 AM
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Default mg/ml to ug/ml dilution

Hi,

I have a stock solution of 550mg/ml that I want to dilute to 50ug/ml

How would I do this?
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  #2  
Old 05-26-2009, 11:14 AM
JohnS JohnS is offline
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Default Re: mg/ml to ug/ml dilution

Quote:
Originally Posted by Unregistered
Hi,

I have a stock solution of 550mg/ml that I want to dilute to 50ug/ml

How would I do this?
Measure out any convenient amount, say 1 mL
Add solvent to make up total of 11000 times that volume, ie 11 liters total. It will be 1/11000 the strength, 50 g/mL.
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  #3  
Old 04-15-2010, 11:04 PM
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Default Re: mg/ml to ug/ml dilution

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Originally Posted by JohnS View Post
Measure out any convenient amount, say 1 mL
Add solvent to make up total of 11000 times that volume, ie 11 liters total. It will be 1/11000 the strength, 50 g/mL.
solution:


1 g/mL= 0.001 mg/ml
50 g/mL = 0.05 mg/ml

stock sol is 550mg/ml,
so take 5 ml from stock soln, and dilute it upto 100 ml. ( u will get 50 g/mL dilution)
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  #4  
Old 04-16-2010, 02:44 AM
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Default Re: mg/ml to ug/ml dilution

Quote:
Originally Posted by Unregistered View Post
solution:


1 g/mL= 0.001 mg/ml
50 g/mL = 0.05 mg/ml

stock sol is 550mg/ml,
so take 5 ml from stock soln, and dilute it upto 100 ml. ( u will get 50 g/mL dilution)
This is incorrect. If you dilute 5 mL to 100 mL, concentration will be 27.5 mg/mL
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  #5  
Old 04-16-2010, 12:54 PM
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Default Re: mg/ml to ug/ml dilution

Quote:
Originally Posted by Unregistered View Post
solution:


1 g/mL= 0.001 mg/ml
50 g/mL = 0.05 mg/ml

stock sol is 550mg/ml,
so take 5 ml from stock soln, and dilute it upto 100 ml. ( u will get 50 g/mL dilution)
Thanks for coming on and helping. Here is where you went wrong:

The original stock solution is VERY concentrated: 550,000ppm.

If you take 5mL, you have 2750mg, which you are diluting to 100mL. This gives a resulting conc of 27500mg/L or 27500ppm. (27.5mg/mL). Still quite concentrated.

Hope this helps.
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  #6  
Old 07-01-2011, 10:49 AM
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Default Re: mg/ml to ug/ml dilution

V1*C1 = V2*C2

V1: The volume of solvent that you'll need to add
C1: The concentration of the initial stock solution
V2: The final volume you want
C2: The concentration you want in your final volume
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  #7  
Old 07-01-2011, 02:05 PM
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Default Re: mg/ml to ug/ml dilution

Quote:
Originally Posted by Unregistered View Post
V1*C1 = V2*C2

V1: The volume of solvent that you'll need to add
C1: The concentration of the initial stock solution
V2: The final volume you want
C2: The concentration you want in your final volume
I think V1 is mislabelled and is the volume of stock solution required.
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  #8  
Old 01-20-2012, 03:28 AM
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Default Re: mg/ml to ug/ml dilution

I have a stock solution of 100mg/ml,then how can i prepare 2mg/ml.
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  #9  
Old 01-20-2012, 09:17 AM
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Default Re: mg/ml to ug/ml dilution

Quote:
Originally Posted by Unregistered View Post
I have a stock solution of 100mg/ml,then how can i prepare 2mg/ml.
Dilute 50:1. Measure out any convenient amount, add solvent to dilut to total volume 50X that amount, for example 1 mL, dilute to 50 mL (requires about 49 mL of solvent). Volumes are not EXACTLY additive so dilute to measured final volume.
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  #10  
Old 10-12-2012, 10:56 AM
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Default Re: mg/ml to ug/ml dilution

Quote:
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I have a stock solution of 100mg/ml,then how can i prepare 2mg/ml.
Please reply immediately
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