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#1
05-26-2009, 10:14 AM
 Unregistered Guest Posts: n/a
mg/ml to ug/ml dilution

Hi,

I have a stock solution of 550mg/ml that I want to dilute to 50ug/ml

How would I do this?
#2
05-26-2009, 11:14 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,539 Rep Power: 19
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered Hi, I have a stock solution of 550mg/ml that I want to dilute to 50ug/ml How would I do this?
Measure out any convenient amount, say 1 mL
Add solvent to make up total of 11000 times that volume, ie 11 liters total. It will be 1/11000 the strength, 50 µg/mL.
#3
04-15-2010, 11:04 PM
 Unregistered Guest Posts: n/a
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by JohnS Measure out any convenient amount, say 1 mL Add solvent to make up total of 11000 times that volume, ie 11 liters total. It will be 1/11000 the strength, 50 µg/mL.
solution:

1 µg/mL= 0.001 mg/ml
50 µg/mL = 0.05 mg/ml

stock sol is 550mg/ml,
so take 5 ml from stock soln, and dilute it upto 100 ml. ( u will get 50 µg/mL dilution)
#4
04-16-2010, 02:44 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,539 Rep Power: 19
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered solution: 1 µg/mL= 0.001 mg/ml 50 µg/mL = 0.05 mg/ml stock sol is 550mg/ml, so take 5 ml from stock soln, and dilute it upto 100 ml. ( u will get 50 µg/mL dilution)
This is incorrect. If you dilute 5 mL to 100 mL, concentration will be 27.5 mg/mL
#5
04-16-2010, 12:54 PM
 Mrs X can't count, can't spell! Join Date: Feb 2006 Location: New Zealand Posts: 2,436 Rep Power: 12
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered solution: 1 µg/mL= 0.001 mg/ml 50 µg/mL = 0.05 mg/ml stock sol is 550mg/ml, so take 5 ml from stock soln, and dilute it upto 100 ml. ( u will get 50 µg/mL dilution)
Thanks for coming on and helping. Here is where you went wrong:

The original stock solution is VERY concentrated: 550,000ppm.

If you take 5mL, you have 2750mg, which you are diluting to 100mL. This gives a resulting conc of 27500mg/L or 27500ppm. (27.5mg/mL). Still quite concentrated.

Hope this helps.
#6
07-01-2011, 10:49 AM
 Unregistered Guest Posts: n/a
Re: mg/ml to ug/ml dilution

V1*C1 = V2*C2

V1: The volume of solvent that you'll need to add
C1: The concentration of the initial stock solution
V2: The final volume you want
C2: The concentration you want in your final volume
#7
07-01-2011, 02:05 PM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,539 Rep Power: 19
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered V1*C1 = V2*C2 V1: The volume of solvent that you'll need to add C1: The concentration of the initial stock solution V2: The final volume you want C2: The concentration you want in your final volume
I think V1 is mislabelled and is the volume of stock solution required.
#8
01-20-2012, 03:28 AM
 Unregistered Guest Posts: n/a
Re: mg/ml to ug/ml dilution

I have a stock solution of 100mg/ml,then how can i prepare 2mg/ml.
#9
01-20-2012, 09:17 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,539 Rep Power: 19
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered I have a stock solution of 100mg/ml,then how can i prepare 2mg/ml.
Dilute 50:1. Measure out any convenient amount, add solvent to dilut to total volume 50X that amount, for example 1 mL, dilute to 50 mL (requires about 49 mL of solvent). Volumes are not EXACTLY additive so dilute to measured final volume.
#10
10-12-2012, 10:56 AM
 Unregistered Guest Posts: n/a
Re: mg/ml to ug/ml dilution

Quote:
 Originally Posted by Unregistered I have a stock solution of 100mg/ml,then how can i prepare 2mg/ml.

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