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Old 04-01-2009, 06:49 AM
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Default Convert 10mg/mL to uM

I have 300mg total of chemotherapy drug, it is diluted in 30mL DMSO for a concentration of 10mg/mL. The MW is 637.04. I want to make up a 20uM stock solution - how do I go about doing this conversion?
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Old 04-01-2009, 07:19 AM
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Default Re: Convert 10mg/mL to uM

Quote:
Originally Posted by Unregistered
I have 300mg total of chemotherapy drug, it is diluted in 30mL DMSO for a concentration of 10mg/mL. The MW is 637.04. I want to make up a 20uM stock solution - how do I go about doing this conversion?
20 ÁM means 20 Ámol/L. The first question is how much solution do you wish to make. Since you didn't say, lets assume 1 L. Then you need 20 Ámol.

The molar mass is 637.04 g/mol, so you need 12741 Ág, of 12.741 mg.

Since it is currently 10 mg/L, you need 1.2741 mL (you may need to round this, depending on how accurately you can measure. Put in a volumetric flask and dilute to 1 L.
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Old 04-27-2010, 09:01 PM
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Default Re: Convert 10mg/mL to uM

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Originally Posted by Unregistered View Post
I have 300mg total of chemotherapy drug, it is diluted in 30mL DMSO for a concentration of 10mg/mL. The MW is 637.04. I want to make up a 20uM stock solution - how do I go about doing this conversion?
CONVERSION OF mg/l to ÁM/l
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Old 04-28-2010, 04:31 AM
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Default Re: Convert 10mg/mL to uM

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CONVERSION OF mg/l to ÁM/l
The specific question was answered in post 2. If you are asking "generally," there is no "general" answer. You HAVE to know the substance and its molecular weight, which is also the molar mass in grams per mole.
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Old 12-18-2010, 09:09 PM
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Default Re: Convert 10mg/mL to uM

Re: Convert 10mg/mL to uM
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Old 04-11-2011, 06:43 PM
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Default Re: Convert 10mg/mL to uM

I have 0.05 mg/mL and need it converted to micromolar. How do I do that?
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Old 04-12-2011, 02:52 AM
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Default Re: Convert 10mg/mL to uM

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I have 0.05 mg/mL and need it converted to micromolar. How do I do that?
Molar is moles per liter. 0.05 mg/ml = 50 mg/L
Now you need to know the molecular weight of the substance and divide by it to convert grams to moles. The part is substance-specific.
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Old 07-24-2012, 05:43 PM
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Default Re: Convert 10mg/mL to uM

i have a stock solution of 1mg/ml benzylaminopurine. the MW of benzylaminopurine is 225.26. how much did i need to make a 1L media with 8.8uM benzylaminopurine?
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Old 07-27-2012, 03:45 AM
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Default Re: Convert 10mg/mL to uM

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i have a stock solution of 1mg/ml benzylaminopurine. the MW of benzylaminopurine is 225.26. how much did i need to make a 1L media with 8.8uM benzylaminopurine?
You need 8.8 Ámol of benzylaminopurine times 225.26 g/mol = 1.9823 mg.
Use 1.9823 mL of stock, dilute to 1 L final volume.
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Old 09-13-2012, 06:46 AM
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Default Re: Convert 10mg/mL to uM

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Originally Posted by JohnS View Post
20 ÁM means 20 Ámol/L. The first question is how much solution do you wish to make. Since you didn't say, lets assume 1 L. Then you need 20 Ámol.

The molar mass is 637.04 g/mol, so you need 12741 Ág, of 12.741 mg.

Since it is currently 10 mg/L, you need 1.2741 mL (you may need to round this, depending on how accurately you can measure. Put in a volumetric flask and dilute to 1 L.
perfect thank you
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