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#1




calculate IU
Hi guys,
I have a solution to prepare but for some reason i am stuck.. I have a vial (1.5ml) with 300 IU/ml FSH.. From that I need to use just a 10 IU/ml. So I need to take from the vial just 50 microliters? this equals 10 IU/ ml, right? In addition, I need to make a solution with 10 IU/ml FSH into PBS, although I do not know the final volume that the FSH/PBS must have.. Although I need just 1ml of the final solution Any help mych appreciated.. Gio 
#2




Re: calculate IU
Quote:
You must withdraw 10 IU from a stronger solution of 300 IU/mL, hence you need 10 IU x 1 mL/300 IU = 33.3 µL of the stronger solution. That must be diluted to a final volume of 1 mL with water, or whatever the appropriate liquid is. You might make an amount that avoids messy fractions after the division 0.9 mL: Use 30 µL, dilute to 0.9 mL 1.2 mL: Use 40 µL, dilute to 1.2 mL Actually, use x, dilute to final volume of 30x Last edited by JohnS; 03022009 at 12:16 PM. 
#3




Re: calculate IU
Thank you very much John

#4




Re: calculate IU
In addition, you would normally make these final solutions up in a volumetric flask, as you can get small volume changes whn mixing different solutions.

#5




How to calculate the enzyme units
hi guys,
after induction i purified the sample and i got the required protein by protein purification technique. i got the purified protein in three elutes. elute1, elute2, elute3. by lowry method i got the value of 0.032mg/ml. i used 5 mL of bacterial culture. I used 150microlitres to elute the sample for three times. May i know how to calculate IU/mL. 
#6




Re: calculate IU
You have 300 IU/cc. You need 10 IU.
It is unusual to have a vial label which does not have the concentration as amount per cc verses amount concentrated as you stated. For a volume this small, I would draw up 0.33 cc (100 IU) and dilute into a total volume of 10 cc, resulting in a concentration of 10 IU/cc. Another way would be to draw up 1.0 cc and dilute to a total volume of 30 cc, resulting in a concentration of 10 IU/cc. Another way would be to draw up 0.5 cc and dilute to a total volume of 15 cc, resulting in a concentration of 10 IU/cc. Regards C Last edited by Carl M; 02282011 at 04:37 PM. 
#7




Re: How to calculate the enzyme units
Quote:

#8




calculate IU
I have TSH 10 UG stock. I need a 100 IU/mL TSH solution. Any Idea how to make this solution.
Arjmand 
#9




Re: calculate IU
What is the volume of the UG? Also, exactly what is UG? IU is international units.

#10




Re: convertion of IU to milligrams
I have a vial consisting of 100mu/vl of penicillin G. I have to prepare a solution with a concentration of 10micrograms/ml. I am stuck at this point. Any help in this regard will be appreciated
Cheers Vasu 
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