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#1
03-02-2009, 10:52 AM
 gio_1980 Guest Posts: n/a
calculate IU

Hi guys,

I have a solution to prepare but for some reason i am stuck..
I have a vial (1.5ml) with 300 IU/ml FSH.. From that I need to use just a 10 IU/ml. So I need to take from the vial just 50 microliters? this equals 10 IU/ ml, right?
In addition, I need to make a solution with 10 IU/ml FSH into PBS, although I do not know the final volume that the FSH/PBS must have.. Although I need just 1ml of the final solution

Any help mych appreciated..
Gio
#2
03-02-2009, 11:21 AM
 JohnS Moderator Join Date: Dec 2007 Location: SE Michigan, USA Posts: 9,445 Rep Power: 19
Re: calculate IU

Quote:
 Originally Posted by gio_1980 Hi guys, I have a solution to prepare but for some reason i am stuck.. I have a vial (1.5ml) with 300 IU/ml FSH.. From that I need to use just a 10 IU/ml. So I need to take from the vial just 50 microliters? this equals 10 IU/ ml, right? In addition, I need to make a solution with 10 IU/ml FSH into PBS, although I do not know the final volume that the FSH/PBS must have.. Although I need just 1ml of the final solution Any help mych appreciated.. Gio
If you need 1 mL of 10 IU/mL solution, then you need 10 IU.

You must withdraw 10 IU from a stronger solution of 300 IU/mL, hence you need
10 IU x 1 mL/300 IU = 33.3 µL of the stronger solution.
That must be diluted to a final volume of 1 mL with water, or whatever the appropriate liquid is.

You might make an amount that avoids messy fractions after the division
0.9 mL: Use 30 µL, dilute to 0.9 mL
1.2 mL: Use 40 µL, dilute to 1.2 mL

Actually, use x, dilute to final volume of 30x

Last edited by JohnS; 03-02-2009 at 12:16 PM.
#3
03-02-2009, 12:15 PM
 gio_1980 Guest Posts: n/a
Re: calculate IU

Thank you very much John
#4
03-03-2009, 11:02 AM
 Mrs X can't count, can't spell! Join Date: Feb 2006 Location: New Zealand Posts: 2,413 Rep Power: 12
Re: calculate IU

In addition, you would normally make these final solutions up in a volumetric flask, as you can get small volume changes whn mixing different solutions.
#5
02-27-2011, 09:21 PM
 Unregistered Guest Posts: n/a
How to calculate the enzyme units

hi guys,
after induction i purified the sample and i got the required protein by protein purification technique. i got the purified protein in three elutes. elute-1, elute-2, elute-3. by lowry method i got the value of 0.032mg/ml. i used 5 mL of bacterial culture.
I used 150microlitres to elute the sample for three times.
May i know how to calculate IU/mL.
#6
02-28-2011, 04:18 PM
 Carl M Junior Member Join Date: Feb 2011 Posts: 7 Rep Power: 0
Re: calculate IU

You have 300 IU/cc. You need 10 IU.

It is unusual to have a vial label which does not have the concentration as amount per cc verses amount concentrated as you stated.

For a volume this small, I would draw up 0.33 cc (100 IU) and dilute into a total volume of 10 cc, resulting in a concentration of 10 IU/cc.

Another way would be to draw up 1.0 cc and dilute to a total volume of 30 cc, resulting in a concentration of 10 IU/cc.

Another way would be to draw up 0.5 cc and dilute to a total volume of 15 cc, resulting in a concentration of 10 IU/cc.

Regards
C

Last edited by Carl M; 02-28-2011 at 04:37 PM.
#7
02-28-2011, 04:22 PM
 Carl M Junior Member Join Date: Feb 2011 Posts: 7 Rep Power: 0
Re: How to calculate the enzyme units

Quote:
 Originally Posted by Unregistered hi guys, after induction i purified the sample and i got the required protein by protein purification technique. i got the purified protein in three elutes. elute-1, elute-2, elute-3. by lowry method i got the value of 0.032mg/ml. i used 5 mL of bacterial culture. I used 150microlitres to elute the sample for three times. May i know how to calculate IU/mL.
IU is a measure of activity, not mass, volume or weight. It is completely dependent on the item being measured.
#8
02-07-2012, 10:07 PM
 Unregistered Guest Posts: n/a
calculate IU

I have TSH 10 UG stock. I need a 100 IU/mL TSH solution. Any Idea how to make this solution.

Arjmand
#9
02-08-2012, 07:05 AM
 Carl M Junior Member Join Date: Feb 2011 Posts: 7 Rep Power: 0
Re: calculate IU

What is the volume of the UG? Also, exactly what is UG? IU is international units.
#10
12-26-2012, 07:20 PM
 Unregistered Guest Posts: n/a
Re: convertion of IU to milligrams

I have a vial consisting of 100mu/vl of penicillin G. I have to prepare a solution with a concentration of 10micrograms/ml. I am stuck at this point. Any help in this regard will be appreciated

Cheers
Vasu

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