OnlineConversion Forums Welcome to OnlineConversion.com Forums

Go Back   OnlineConversion Forums > Main Forums > Convert and Calculate

Convert and Calculate Post any conversion related questions and discussions here. If you're having trouble converting something, this is where you should post.
* Guest Posting is allowed.

Reply
 
Thread Tools Display Modes
  #1  
Old 01-07-2009, 07:54 AM
Benchbuddy
Guest
 
Posts: n/a
Smile converting micrograms to nanomolar

Hi!

I am having trouble with a simple calculation. I have purified a recombinant protein (85.83KDa) and it's concentration is 0.19ug/ul (micrograms per microlitre). I used 10ul of this in an experiment and need to know this concentration in nM (nanomolar)?

As a side question, the tag (42.5KDa) is still attached to the purified protein so the entire recombinant protein is 128.33KDa, should I use this value for working out the concentration of protein used in nanomolars in my experiment?

Thank you in advance
Reply With Quote
  #2  
Old 01-08-2009, 04:35 AM
JohnS JohnS is offline
Moderator
Long Time Member The Golden Calculator Award Over 5000 Posts
 
Join Date: Dec 2007
Location: SE Michigan, USA
Posts: 9,551
Rep Power: 19
JohnS will become famous soon enough
Default Re: converting micrograms to nanomolar

Quote:
Originally Posted by Benchbuddy
Hi!

I am having trouble with a simple calculation. I have purified a recombinant protein (85.83KDa) and it's concentration is 0.19ug/ul (micrograms per microlitre). I used 10ul of this in an experiment and need to know this concentration in nM (nanomolar)?

As a side question, the tag (42.5KDa) is still attached to the purified protein so the entire recombinant protein is 128.33KDa, should I use this value for working out the concentration of protein used in nanomolars in my experiment?

Thank you in advance
I was hoping our chemist, Mrs X, would see and answer this. But I'll try.

The question on the tag reduces to whether the stated concentration, 0.19 痢/無 includes the weight of the tag or not, and I don't know. I am assuming it does not. The molecular weight in daltons is the molar mass in grams per mole.

There is also some confusion over whether you want nanomoles or nanomolar. Molar is a term used to describe a concentration in moles per liter. If you use a specific number of liters, then you use a specific number of moles.
0.19 痢/無 is also 0.19 g/L. The molar concentration is
0.19 g/L x 1 mol/85.83 kg = 2.2 痠ol/L or 2.2 然.

You used 10 無 of it, so you used 22 pmol. The other way to get to the same point is 10 無 contains 1.9 痢. Dividng by the molar mass, 22 pmol.
Reply With Quote
  #3  
Old 01-08-2009, 02:06 PM
Mrs X Mrs X is offline
can't count, can't spell!
Long Time Member The Golden Calculator Award The Tree House Award Over 1000 Posts
 
Join Date: Feb 2006
Location: New Zealand
Posts: 2,444
Rep Power: 12
Mrs X will become famous soon enough
Default Re: converting micrograms to nanomolar

Sorry, I didn't see this until now, but what JohnS said.

However, are you taking the original 10無, and diluting it to a different volume? - If so, then you may need to know the concentration in the resulting solution. If you can't work this our from what johnS has written, post back, and someone may be able to help. (I'm going to be away for a few days, I love summer!)
Reply With Quote
  #4  
Old 09-10-2012, 05:56 PM
Unregistered
Guest
 
Posts: n/a
Default How to make 50 ul of 5 nM NaCl

I want to prepare 50 ul of 5 nM NaCl, plz guide
Reply With Quote
  #5  
Old 09-21-2012, 07:04 AM
Omega- SM
Guest
 
Posts: n/a
Default Re: converting micrograms to nanomolar

I need help with a calculation. Forgive me I am far removed from general chem.
I have a substance with a molecular weight of 99.1 kDa. I want to get it into solution at a concentration of .8 nmol/L. How many micrograms do I need to use per microliter.
Thanks
Reply With Quote
  #6  
Old 09-21-2012, 10:00 AM
JohnS JohnS is offline
Moderator
Long Time Member The Golden Calculator Award Over 5000 Posts
 
Join Date: Dec 2007
Location: SE Michigan, USA
Posts: 9,551
Rep Power: 19
JohnS will become famous soon enough
Default Re: converting micrograms to nanomolar

Quote:
Originally Posted by Omega- SM View Post
I need help with a calculation. Forgive me I am far removed from general chem.
I have a substance with a molecular weight of 99.1 kDa. I want to get it into solution at a concentration of .8 nmol/L. How many micrograms do I need to use per microliter.
Thanks
99.1 kg/mol x 0.8 nmol/L = 79.28 痢/L
If you really only want to make a microliter, 79.28 pg/無, but I don't know how you weigh it out.
Reply With Quote
Reply

Thread Tools
Display Modes

Posting Rules
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

Forum Jump


All times are GMT -8. The time now is 07:59 PM.


Powered by vBulletin® Version 3.8.7
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 1997-2005, 2007, OnlineConversion.com