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#21
09-17-2012, 12:24 PM
 JohnS Double Ultimate Supreme Member Join Date: Dec 2007 Location: SE Michigan, USA Posts: 8,706 Rep Power: 17
Re: ug/ml

Quote:
 Originally Posted by Unregistered how much do i need to reconstitute blasticidin S, 50 mg powder to a 15ug/ml solution. MW: 458.9 thanks!
You don't need MW for this calculation.

How much do you want to make? 15 µg/mL is also 15 mg/L. If you wish to use all 50 mg, you can make 50 mg x 1 L/15 mg = 3.33 L.

If you wish to make less, you need to be able to weigh out part of the 50 mg precisely. Multiply the volume (in liters) you wish to make by 15 mg/L. That is the weight you need to measure out.

1 L would require 15 mg, 0.1 L (100 mL) would require 1.5 mg, etc.
#22
05-18-2013, 09:06 AM
 Unregistered Guest Posts: n/a
Re: ug/ml

What does 10ug/mL mean?? Like if I need to check the activity of some compound or extract at this concentration, then how much amount of extract or compound should I make as stock solution?
#23
05-18-2013, 01:04 PM
 Mrs X can't count, can't spell! Join Date: Feb 2006 Location: New Zealand Posts: 2,294 Rep Power: 11
Re: ug/ml

Quote:
 Originally Posted by Unregistered What does 10ug/mL mean?? Like if I need to check the activity of some compound or extract at this concentration, then how much amount of extract or compound should I make as stock solution?
10µg/mL means ten micrograms per millilitre of solution.

There are 1000ng in 1 µg, 1000µg in 1 g, 1000g in 1kg, 1000kg in 1tonne etc. There are 1000µL in 1 mL, 1000mL in 1L, 1000L in 1 cubic meter etc.

If you have 10ng/µL; 10µg/mL, 10mg/L; 10g/m3, you have the same concentration in each solution.

A stock solution is a more concentrated solution you keep in the lab so that you can take portions out to dilute for use. Make up the amount you need for the time you want to use it over.

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