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Unregistered
05-03-2006, 06:53 PM
hi i need help with some conversions and calculations on a pharmacology prac.
i need to find a concentration from a standard curve that i constructed. from the graph the first concentration was read 0.056 (im assuming microMole). from this do i just multiply it by the dilution factor?
next i need help on finding the product amount. we were given the statement "to determine the product amount (in nmol) you need to determine the amount of standard in your final reaction (3ml). be careful to convert the volume of standard used ie. 10ul of a 100uM standard"
how do i do this???

Mrs X
05-04-2006, 12:07 PM
hi i need help with some conversions and calculations on a pharmacology prac.
i need to find a concentration from a standard curve that i constructed. from the graph the first concentration was read 0.056 (im assuming microMole). from this do i just multiply it by the dilution factor?
next i need help on finding the product amount. we were given the statement "to determine the product amount (in nmol) you need to determine the amount of standard in your final reaction (3ml). be careful to convert the volume of standard used ie. 10ul of a 100uM standard"
how do i do this???

Hi, You really need to talk to your tutors about this. If they are not covering this in lectures, then they aren't teaching you properly, or assuming too much prior knowledge.

For the first part, if you are sure you have drawn the calibration curve correctly, then 0.056(umol?) is the amount of (umol?) per litre in that solution. So yes, just work back through your dilution series to get the original amount. And don't assume umol, check it :) This really matters for pharmocology.

For the second part, they are just warning you that you have umol and nmol solutions. Be careful with your orders of magnitude. Good luck.